Cell lines recapitulating physiological processes can represent alternatives to animal or human studies. The 3T3-L1 cell line is used to mimic adipocyte function and differentiation. Since transfection of 3T3-L1 cells is difficult. we used a modified 3T3-L1 cell line overexpressing Cas9 for a straightforward generation of gene knock-outs. https://www.chiggate.com/devacurl-arc-angel-gel-maximum-hold-no-crunch-styler-32oz-for-cheap/